The Zenoquick-Competent E. coli Transformation Kit & Buffer Set are simple methods for generating Zenoquick-Competent E. coli for rapid, reliable and highly efficient DNA transformation. The methods eliminate the requirement of heat-shocking and related procedures. Instead, transformation can be performed by adding DNA to prepared Zenoquick-Competent cells and the mixture spread directly to a culture plate - Mix & Go! Transformation efficiencies typically range from 108 - 107 transformants/μg of pUC19 DNA, but can vary depending on the strain of E. coli. Most E. coli strains respond well to the Zenoquick-Competent preparation method and demonstrate fast transformation kinetics. The procedures are easy. Simply culture the E. coli strain of your choice in Zenoquick Broth medium (or SOB), wash and then resuspend the cells in the provided uniquely formulated buffers. The cells are now ready for transformation.
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