Nitrocellulose and PVDF membranes that have been probed by Western blotting procedures and detected by chemiluminescent or other non-precipitating substrates may be stripped and reprobed using West-Ez Stripping Buffer. Traditional stripping methods use conditions that are effective for only low-affinity antibody-antigen interactions or are so harsh conditions that they tend to adversely alter the antigen for subsequent immunoprobing.
West-Ez Stripping Buffer provides a generally robust but gentle method for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane.
Store at 2-8℃
|Formulation is more efficient at stripping antibodies than homemade buffers
|Does not damage the target antigen during stripping allowing efficient reprobing
|Less expensive than other commercial stripping buffers
|Western Blotting Reagnet