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Nitrocellulose and PVDF membranes that have been probed by Western blotting procedures and detected by chemiluminescent or other non-precipitating substrates may be stripped and reprobed using West-Ez Stripping Buffer. Traditional stripping methods use conditions that are effective for only low-affinity antibody-antigen interactions or are so harsh conditions that they tend to adversely alter the antigen for subsequent immunoprobing.
West-Ez Stripping Buffer provides a generally robust but gentle method for stripping primary and secondary antibodies from blots to enable several reprobings on the same membrane.
Store at 2-8℃
|Effective||Formulation is more efficient at stripping antibodies than homemade buffers|
|Gentle||Does not damage the target antigen during stripping allowing efficient reprobing|
|Economical||Less expensive than other commercial stripping buffers|
|Application||Western Blotting Reagnet|