RPMI-1640 was developed in 1966 by Moore and his co-workers at Roswell Park Memorial Institute (hence the acronym RPMI). It is based on the RMPI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.
While it was originally formulated to support lymphoblastoid is suspension culture, it has been proven to support a wide variety of anchorage-dependant cells. It has a wide range of uses, including the culture of fresh human lymphocytes, fusion protocols, and in the growth of hybrid cells.
- With L-Glutamine
- Without HEPES
Specification
| D-Glucose |
2000 mg/L |
| L-Glutamine |
300 mg/L |
| HEPES |
No |
| Phenol Red |
5 mg/L |
Store at 2-8℃
