The RIPA Buffer is a reliable buffer used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. It enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the BCA Protein Assay, immuno-assays and protein purification.
Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail are also compatible with the RIPA Buffer and can be added before use to prevent proteolysis and maintain protein phosphorylation.
- Ready-to-Use Solution
- Lyse Cultured Mammalian Cells
- Minimizes Non-Specific Protein Binding
- Radioimmunoprecipitation Assay Buffer
- Low Background
Components
RIPA Cell Lysis Buffer(1X) With EDTA - 100ml,contains 150mM Sodium Chloride, 1% triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 50mM Tris-HCl, pH7.5, and 2mM EDTA, sterile solution.
Application
Suitable for molecular biology protein chemistry and biochemical applications.
Store at 2-8℃
