Affinity purified antibody isolated from a pool of
serum from rabbits immunized with purified sheep
IgG was labeled with peroxidase by the periodate
method of Nakane and Kawaoi.
ELISA:1/1000 - 1/5000(1.0 ug/mL - 0.2 ug/mL)
Blotting:1/5000- 1/20000(0.2 ug/mL - 0.05 ug/mL)
Histo/Cytochemical Procedures:1/200 - 1/500
(5.0 ug/mL - 2.0 ug/mL)
Note: In many cases, the antibody may be
diluted further than indicated
Concentration: 1mg/ml
Store at 2-8℃
| Specificity/Cross Reactivity |
Tested by gel diffusion and ELISA techniques as applicable. This product reacts specifically with sheep IgG and may recognize other immunoglobulin types that have light chains in common with IgG. Reactivity to IgG subclasses has not been tested. Antibodies to sheep IgG may cross-react with immunoglobulins of other mammalian species if common binding sites are shared. |
| Suggested Working Concentration |
Optimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution. |
| Application |
ELISA,Blotting,Histo/Cytochemical Procedures |
