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Protein G is a bacterial cell wall protein and binds to most mamalian immunogoblulins primarily through their Fc regions. Native Protein G contains two immunogoblin binding sites, as well as albumin biding sites.
Puredown Protein G is covalently coupled to highly cross-linked agarose bead.This product is provided preblocked with fatty acid free bovine serum albumin and have been extensively washed to reduce non-specific binding. Formulations are ready to use and 10ul of agarose per 1ug of primary antibody can be used in standard immunoprecipitation procedues.
|Reduced Background||Minus albumin-binding region to reduce non-specific binding|
|Convenient||Ready to use for immunoprecipitation procedures|
|Low Cost||Cost less than other competitor Protein A/G-Agarose|
|Concentration||4% cross-linked agarose|
|Binding Capacity||> 18mg/ml Human IgG|
Store at 2-8℃
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