Protein A is a cell wall component and four high affinity binding sites capable of binding specificaly to the Fc region of immunoglobin molecules from several species. The Protein A molecule is heat-stable and retains its native conformation, when exposed to denaturing reagents such as 4M urea, 4M thiocynate and 6M guanidine hydrochloride.
Puredown Protein A is covalently coupled to highly cross-linked agarose bead. This product is provided preblocked with fatty acid free bovine serum albumin and have been extensively washed to reduce-non-specific binding. Formulations are ready to use and 10ul of primary antibody can be used in standard immuno-precipitaion procedures.
Specification
| Reduced Background |
Minus albumin-binding region to reduce non-specific binding |
| Convenient |
Ready to use for immunoprecipitation procedures |
| Low Cost |
Cost less than other competitor's Protein A/G-Agarose |
| Concentration |
4% cross-linked agarose |
| Binding Capacity |
> 35mg/ml Human IgG |
| Application |
Immunoprecipitation (IP) |
Store at 2-8℃
