Downstream purification is the leading disadvantage to using serum in cell culture media. For example, monoclonal antibody production and recombinant protein secretion both require purification to remove serum-derived gamma globulin from the media.
Insulin-Transferrin-Selenium Solution II(100X) , is a growth supplement that should be added at 10 mL/L medium. Supple-mentation enables a reduction in the FBS requirements of the culture. The components of ITS are required for optimal perfor-mance of cells in serum-free culture. Some other trace elements and nutrients are contained additionally.
All components are derived from non-animal source
Recombinant HAS : 0.05g/L
Recombinant Insulin ( 28 units/mg) : 0.5g/L
Recombinant Transferrin : 0.6g/L
Sodium Selenite : 0.00067g/L
Ethanolamine : 0.01g/L
CuSO4 : 0.0000016g/L
ZnSO4 : 0.000863g/L
Ferric citrate : 0.00204g/L
Chemically Defined Nutrients : 0.16g/L
Store at 2-8℃
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