Ham's F-10 and F-12 nutrient mixtures were both originally developed to support the growth of a variety of cells, particularly Chinese Hamster Ovary (CHO) cells, with or without serum supplementation. Ham's F-10 has since been used in the culture of a wide variety of cells of both animal and human origin. Ham's F-12 is prefered by many in the culture of cells of rodent origin. It has also shown excellent results as a cloning medium for myeloma and hybridoma cells.
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