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Affinity purified antibody isolated from a pool of
serum from goats immunized with purified chicken
IgY was labeled with peroxidase by the periodate
methodof Nakane and Kawaoi.
Store at 2-8℃
|Specificity/Cross Reactivity||Tested by gel diffusion and ELISA techniques as applicable. This product reacts specifically with chicken IgY and may recognize other immuno-globulin types that have light chains in common with IgG. Reactivity to IgG subclasses has not been tested. Antibodies to chicken IgY may cross-react with immunoglobulins of other avian species if common binding sites are shared.|
|Suggested Working Concentration||Optimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution.|
|ELISA||1:500 - 1:2500 (1.0 μg/mL - 0.2 μg/mL)|
|Western Blot||1:2500 - 1:10000 (0.2 μg/mL - 0.05 μg/mL)
|Histo/Cytochemical||1:100 - 1:250 (5.0 μg/mL - 2.0 μg/mL)|
|Application||ELISA, Blotting, Histo/Cytochemical|
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