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G418 disulfate salt is an aminoglycoside antibiotic similar in structure to gentamicin used for selection and maintenance of prokaryotic and eukaryotic cells transfected with a iNOS promoter construct and neomycin resistance gene. Optimal concentrations for selection and maintenance vary by cell lines. Bacteria and algae require 5 μg/mL or less while animal cells may require 300-500 μg/mL
Store at RT
|Biochem/physiol Actions||G-418 inhibits protein synthesis at the early stages of elongation, post-initiation, at the initiation stage of translation as well as just before the initiation stage of translation.|
|Solubility||H2O: 100 mg/mL (As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 8 days.)|
|Potency||≥450 μg per mg (Dried basis)|
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