Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast. Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials. Bovine pancrease is a rich source of RNase A which is often found in many commerical DNase preparations.
Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNas greatly facilitates purification of an enzyme with undetected levels of recomninant DNase I are completely devoid of aminal based components which elimiates the possibility of introducing animal derived pathogens into bioprocessing procedures.
Store at -20℃
|Deoxyribonuclease I, RNase Free , 2 units/ul in 5mM Calcium acetate, 4mg/ml glycine,and 50% Glycerol pH 5.0
10X DNase I Reaction Buffer,
500mM Tris-HCl, 10mM MgSO4, 1mM CaCl2, pH 7.8.