|1 unit causes an increase in absorbance at 260nm of 0.0001 per minute per ml at 25oC when acting upon highly polymerized DNA at pH 5.0.
Note: As measured at GenDEPOT, one Kunitz unit digests 1ug of lambda DNA in 10 minutes at 37oC in 50mM Tris, 1mM Mg2+, 1mM Ca2+, pH 7.8 in a 50ul reaction. Correlation of digestion units with Kunitz units is different for other DNA and buffer systems.
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