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amfiXpand Taq DNA Polymerase provides superior yield in both routine and challenging PCR application. amfiXpand Taq DNA Polymerase is composing of a unique enzyme blend containing two thermo stable polymerases: Taq DNA Polymerase and a thermostable polymerase with proofreading activity. amfiXpand Taq DNA Polymerase is designed to amplify fragments up to 5kb with high yield and fidelity, as well as with high specificity from episomal and genomic DNA. In addtion a amfiXpand 2X Reaction Buffer including dNTPs and Mg++ minimizes reagent handling steps and reduces the risk of contamination.
For added convenience, amfiXpand Taq DNA Polymerase contains red and yellow loading dyes to allow loading PCR product directly on a gel after thermal cycling, minimizing pipetting steps and providing easy visualization of sample. The red dye runs in a range between 500bp (2% gel) and 1,500bp (0.8% gel) and the yellow dye runs at less than 10bp. amfiXpand 2X Reaction buffer contains fixed MgCl2 concentration of 1.75mM. However, higher concentrations may be achieved by adding additional MgCl2.
Note: MgCl2 is included in the 2X Reaction Buffer at a final concentration of 1.75mM, which is sufficient for most targets. For some targets, more Mg²⁺ may be required.
Store at -20℃
Store at -20℃
|Unique Enzyme Blend||amfiXpand PCR Master Mix is composed of a unique enzyme blend containing two thermostable polymerases : Taq DNA polymerase and a thermostable polymerase with proofreading acitivity.|
|Direct Loading||Load directly to gel from thermocycler No additional loading buffer needed Separates into 2 colors during gel electrophoresis|
|Contents||amfiXpand Taq DNA Polymerase, 1u/ul
amfiXpand 2X Reaction Buffer
|Composition for each reaction||1u/rxn amfiXpand Taq DNA Polymerase, Reaction Buffer,1.75mM MgCl2, 0.2mM dGTP, 0.2mM dATP, 0.2mM dTTP, 0.2mM dCTP, stabilizers, and red and yellow loading dyes.|
|Unit Definition||One unit is defined as the amount of the enzyme required to catalize the incorporation of 10nmol of dNTPs into acid-insoluble material in 30 min at 74 ℃|
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