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amfiXpand Hot-Start Version DNA Polymerase is designed for hot start PCR, a techinique that enhances the specificity, sensitivity and yield of DNA amplification. In addition, the enzyme provides the convenience of reaction set-up at room temperature. In general, hot start PCR methods reduce or eliminate non-specific primers may bund non-specifically, which often leads to unwanted amplification products and primer-dimers. In order to resolve this problem, amfiXpand 2X Hot-Start PCR Buffer includes a DNA binding protein that is especially useful at blocking primers at lower temperatures making them unavailable for use by a polymerase. This DNA binding protein effectively blocks DNA synthesis from mis-priming events at lower temperatures.
amfiXpand Hot-Start Version DNA polymerase is an antibody-inactivated DNA polymerase that can be introduced into PCR protocols with no modification to cycling parameters and inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers. The functional activity of the enzyme is restored during a short 4-minute incubation at 95oC.
Store at -20℃
Store at -20℃
|Contents||amfiXpand Hot-Start Version DNA Polymerase,2units/ul
amfiXpand 2X Hot-Start PCR Buffer
|Hot Start PCR||Hot start PCR methods reduce or eliminate non-specific primer-extension products formed at lower temperatures during assembly of PCR reactions|
|Direct Loading||Load directly to gel from thermocycler No additional loading buffer needed Separates into 2 colors during gel electrophoresis|