amfiSure Ultra Fidelity PCR Master Mix is ready-to-use mixtures that include all of the reagents needed for proof-reading PCR. amfiSure Ultra Fidelity PCR Master Mix contains modified Pfu, which is 0.25x10-6 errors per cycle, which is 100-fold lower than Taq DNA polymerase. It generate blunt-end PCR product.
amfiSure Ultra Fidelity PCR Master Mix can extend a kilobase of sequence in 15 seconds. amfiSure Ultra Fidelity PCR Master Mix contains MgCl2 (1.5 mM ) at the final 1x reaction concentration. Reactions can be further optimized using the provided DMSO or MgCl2.
This optimized enzyme mixture allows efficient amplification of up to 20kb from human genomic DNA.
Store at -20℃
| PCR Speed |
amfiSure Ultra PCR Master Mix only needs 15 seconds for each Kb in extension step |
| Fidelity |
amfiSure Ultra Fidelity PCR Master Mix has an accuracy 100 times higher than Taq DNA Polymerase and 10-fold higher than Pfu DNA Polymerase |
| Application |
PCR |
| Cloning |
| Long or Difficult Amplification |
| High-Throughput PCR |
| Mutation Analysis |
