amfiSure qGreen 1-Step RT-qPCR Master Mix is a optimized reagent for one-step real-Time qPCR using qGreen. The enzyme mix includes amfiXpand Fast Taq DNA Polymerase, amfiRivert II Reverse Transcriptase, RNase inhibitor and stabilizer. The buffer mix includes dNTPs, MgCl2, and enhancing additives in an optimized buffer, so a reaction can be started simply by adding the template sample and primers for detecting the desired target. The quick and simple protocol allows the reverse transcription and qPCR reactions to be performed in the same tube.
The reverse transcription reaction uses the novel amfiRiert II Reverse Transcriptase, which displays increased heat tolerance (up to 55o) while maintaining the
specificity and extensibility of amfiRivert Transcriptase. This allows cDNA synthesis from RNA with a more complex secondary structure. After cDNA synthesis,
amfiXpnad Fast Taq performs highly specific and efficient PCR amplification, while the fluorescence emitted by qGreen(substitute for SYBR Green I dye) is detected
in real-Time. amfiSure qGreen 1-Step RT-qPCR Mix is also highly resistant to a wide variety of inhibitory substances such as heparin (blood) and humic acid,
allowing stable one-step real-Time RT-qPCR to be performed on a wide range of samples. This product can be used for various applications such as gene expression, RNA virus detection, etc.
Store at -20℃
|amfiSure qGreen 1-Step RT-qPCR Enzyme Mix(20X)
amfiSure qGreen 1-Step RT-qPCR Buffer Mix (2X)