amfiSure ONE Taq DNA Polymerase provides superior yield in both routine and challenging PCR application. amfiSure ONE Taq DNA Polymerase is composed of a unique enzyme blend containing two thermo stable polymerases: Fast Taq DNA Polymerase and high fidelity enhanced polymerase with proofreading activity.
amfiSure ONE Taq DNA Polymerase contains single stranded DNA binding protein based hot start function that enhances the specificity and yield of DNA amplification. amfiSure ONE Taq DNA Polymerase amplify fragments up to 20kb with high yield and fidelity,as well as with high specificity from episomal and genomic DNA.
For added convenience, amfiSure ONE Taq DNA Polymerase contains red and orange loading dyes to allow loading PCR product directly on a gel after thermal cycling, minimizing pipetting steps and providing easy visualization of sample. The red dye runs in a range between 500bp (2% gel) and 1,500bp (0.8% gel) and the orange dye runs at less than 10bp. amfiSure ONE 2X Reaction buffer contains fixed MgCl2 concentration of 1.75mM and dNTP mixture. However, higher concentrations may be achieved by adding additional MgCl2.
Store at -20℃
| Components |
amfiSure ONE Taq DNA Polymerase, 1unit/ul
amfiSure ONE 2X Reaction Buffer |
| Unique Enzyme Blend |
amfiSure ONE Taq DNA Polymerase is composing of a unique enzyme blend containing two thermo stable polymerases: Fast Taq DNA Polymerase and high fidelity enhanced polymerase with proofreading activity. |
| Robust PCR |
Amplify up to 20Kb Genomic DNA |
| Application |
Robust Routin PCR |
| Mouse tail Genotyping PCR |
| GC-Rich PCR |
| Multiplex PCR |
| Colony PCR |
