amfiRivert Sensi cDNA Master Mix (4X) is specially designed for high-yield Reverse transcription system that provides all the components needed for cDNA synthesis. The master mix produces excellent results in both real-time and conventional RT-PCR. amfiRivert Sensi cDNA Master Mix (4X) is fully active at 45-55 oC, providing the higher cDNA yields and successful cDNA synthesis for difficult RNA transcription.
amfiRivert Sensi cDNA Synthesis Master Mix (4X) includes an optimized primer mix which results in the generation of first-strand cDNAs from an entire transcript without the end-bias observed with typical oligo (dT) or random hexamer primers. This mixed primer strategy overcomes variability in real-time PCR gene expression analysis that can result from using different individual primers.
For convenience, amfiRivert Sensi cDNA synthesis Master Mix (4X) contains a amfiRivert Reverse Transcriptase, RNase Inhibitor Plus, MgCl2 and dNTP Mixtures, and optimized primer mix
(Oligo (dT) 18 Random Hexamer) minimizing extra optimization and pipetting steps.
Store at -20℃
| Convenience |
Ready-to-use mixtures that include RT, RNase Inhibitor Plus, dNTP mix, MgCl2 , Oligo dT(18) and Random Hexamer. |
| Eliminates End-bais |
Optimized primer mix which results in the generation of first-strand cDNAs from an entire transcript without the end-bias observed with typical oligo(dT)n or random hexamer primers.
This mixed primer strategy overcomes variability in real-time PCR gene expression analysis that can result from using different individual primers. |
| Specificity |
Fully active 40-60oC, providing increase specificity and the highest cDNA yield |
| Application |
cDNA synthesis reagent |
