IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. The IPTG is used to induce the expression of cloned genes which are under control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.
Store at -20℃
|100mM Stock Solution
|Blue/white colony screening
|Induction of lac operon of protein expression
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