Affinity purified antibody isolated from a pool of
serum from goats immunized with purified chicken
IgY was labeled with peroxidase by the periodate
methodof Nakane and Kawaoi.
Store at 2-8℃
| Specificity/Cross Reactivity |
Tested by gel diffusion and ELISA techniques as applicable. This product reacts specifically with chicken IgY and may recognize other immuno-globulin types that have light chains in common with IgG. Reactivity to IgG subclasses has not been tested. Antibodies to chicken IgY may cross-react with immunoglobulins of other avian species if common binding sites are shared. |
| Suggested Working Concentration |
Optimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution. |
| ELISA |
1:500 - 1:2500 (1.0 μg/mL - 0.2 μg/mL) |
| Western Blot |
1:2500 - 1:10000 (0.2 μg/mL - 0.05 μg/mL)
|
| Histo/Cytochemical |
1:100 - 1:250 (5.0 μg/mL - 2.0 μg/mL) |
| Application |
ELISA, Blotting, Histo/Cytochemical |
