The Xpert Phosphatase Inhibitor Cocktail Solution(100X)is a mixture of several inhibitors to prevent valuableproteins from dephospholylation. Phosphorylation and dephosphorylation is a molecular on/off switch that regulates a number of key biological pathways within the cell, including signal transduction, cell division, and apoptosis.
The Xpert Phosphatase Inhibitor Cocktail preserves phosphorylation of proteins in cell lysates and tissue extracts. The cocktail contains a mixture of our phosphatase inhibitors of broad specificity, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and sodium glycerophosphate. Unlike other commercially available phosphatase inhibitor cocktails which protect against either Ser/Thr phosphatases or protein tyrosine phosphatases.
The Xpert Phosphatase Inhibitor Cocktail protects phosphoproteins from both families of phosphatases. Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is compatible with common protein assays, including the BCA Protein Assay and Bradford-based assays such as Coomassie.
- Ready-to-use stock solution(100X)
- Compatible with standard protein assays
- No resuspention required. User friendly
- Stable up to 2 years
Application
- Each vial is sufficient for a volume of 100ml solution
- Just add to your cell lysis buffer
Store at 2-8℃