Epitope tags provide a method to localize gene products
in a variety of cell types, study the topology of proteins
and protein complexes, identify associated proteins, and
characterize newly identified, low abundance or poorly
immunogenic proteins when protein specific antibodies
are not available. Expression of recombinant proteins
in E-coli as a fusion protein with neighboring histidine
residues is one of the most popular methods of epitope
tags.
The affinity of the histidine-tag motif to Ni2+ by chelation
is strong and selective enough to enable purification of
the protein to homogeneity by affinity chromatography
on a Ni2+ -NTA adsorbent.
Store at -20℃
| Reactivity |
Human |
| Clonality |
Monoclonal , HIS.H8 |
| Host |
Mouse |
| Isotype |
IgG2b |
| Form |
Liquid, 1mg/ml in 10mM PBS, pH 7.2 with 0.05% NaN3 |
| Application |
WB - 1:1000 ~ 1:3000 |
| IP - 1:50 ~ 1:200 |
| IF/ICC - 1:200 ~ 1:500 |
