Protein G is a bacterial cell wall protein and binds to most mamalian immunogoblulins primarily through their Fc regions. Native Protein G contains two immunogoblin binding sites, as well as albumin biding sites.
Puredown Protein G is covalently coupled to highly cross-linked agarose bead.This product is provided preblocked with fatty acid free bovine serum albumin and have been extensively washed to reduce non-specific binding. Formulations are ready to use and 10ul of agarose per 1ug of primary antibody can be used in standard immunoprecipitation procedues.
Specification
| Reduced Background |
Minus albumin-binding region to reduce non-specific binding |
| Convenient |
Ready to use for immunoprecipitation procedures |
| Low Cost |
Cost less than other competitor Protein A/G-Agarose |
| Concentration |
4% cross-linked agarose |
| Binding Capacity |
> 18mg/ml Human IgG |
Store at 2-8℃
