Lysozyme is an enzyme capable of breaking down the bacterial cell wall, and is typically used to enhance protein extractions or nucleic acid extractions. It works by hydrolyzing the 1,4-β-linkages located between the N-acetylmuramic acid and the N-acetyl-D-glucosamine residues in peptidoglycan.
Gram-positive cells have an increased susceptibility to the actions of lysozyme since gram-positive cells have a higher quantity of peptidoglycan in the cell wall. Gram-negative cells are less vulnerable, but can still be hydrolyzed in the presence of EDTA.
Enzymatic Activity : > 45,000 FIP U/mg
1 FIP-Unit equals the amount of enzyme which reduces the absorbance by
0.001 per min. at 450 nm, 25 °C, pH 7.00 using a suspension of FIP Micrococcus
luteus as substrate (acc. to Pharmaceutical Enzymes 1997). 1 FIP-Unit resembles
approximately 1 Shugar-Unit.
Store at -20℃
