A major application for Ribonuclease A (RNase A) is the removal of RNA from preparations of plasmid DNA. In this application, the presence of DNase activity as an impurity is a concern. The boiling-water bath method used to eliminate contaminating DNase activity has proven unreliable. For this reason, GenDEPOT developed a proprietary chromatographic preparation method for elimination of DNase activity. RNase A is an endoribonuclease that attacks at the 3’ phos -phate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single
stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges.In contrast to RNase B, it is not a glycoprotein. RNase A can be inhibited by alkylation of His¹² or His¹¹⁹, which are present in the active site of the enzyme.
Store at 2-8℃
| Unit Definition |
One Kalnitsky Unit(KU) causes an increase in absorbance of 1.0 at 260nm at 37°C and pH 5.0 when yeast ribosomal RNA is hydrolyzed to acid soluble oliognucleotides. One KU unit equals 50 units. |
