The PURY Plasmid Maxi Kit is designed for efficient isolation of plasmid DNA from E. Coli using a traditional 3-buffer procedure that is simple, rapid, user-friendly, and reliable. This Product is designed for preparation of up to 300-500 ug of transfection grade DNA using PURY Maxi-Tip.
PURY Plasmid Maxi-Tip protocols are based on a modified alkaline lysis procedure, followed by binding of plasmid DNA to resin under appropriate low-salt and pH conditions. RNA, proteins, dyes, and low-molecular–weight impurities are removed by a medium-salt wash. Plasmid DNA is eluted in a high-salt buffer and then concentrated and desalted by isopropanol precipitation.
Each disposable PURY Maxi-Tip packed with resin is designed to operate by gravity flow, reducing the amount of hands-on time required for the purification procedure.