Proteinase K is a non-specific serine protease having a very high specific activity. It is used to purify target material from contaminating proteins, for the isolation of mRNA or high molecular weight DNA and to inactivate other enzymatic activities.
Proteinase K is active with or without the presence of SDS, EDTA and urea. This proteinase K is isolated from Tritirachium album and it is very stable in the storage buffer at +4 °C and at –20 °C.
Storage Buffer
Proteinase K in storage buffer composed of 20mM Tris-HCl(pH 7.4), 1mM CaCl2 and 50% glycerol.
Unit Definition
One unit of enzyme liberates Folin-positive amino acid peptides corresponding to 1umol tyrosine in 1min at 37 ℃ using denatured hemoglobin as substrate.
Store at -20℃
