This high sensitivity liquid substrate for peroxidase consists of Tetramethylbenzidine (TMB) plus dilute hydrogen peroxide in a single-reagent stabilized form. The reagent has been specifically formulated for measuring peroxidase in ELISA systems which is stable for long-term storage providing sensitivity greater than OPD.
This reagent is available as a single component, ready-to-use reagent. When used in conjunction with our proprietary TMB Stop Buffer (TSB), the resulting color product will not fade or darken for several hours.
Store at 2-8℃
Peak Wavelengths |
650 nm, unstopped, blue reaction product 450 nm, stopped, yellow reaction product |
Reaction Temperature |
Room temperature |
Substrate Concentration |
2.08 mmol/L |