Pfu DNA Polymerase Ultra Pure is a thermostable enzyme that replicates DNA at 75°C. It catalyzes the polymerization of nucleotides into duplex DNA in the 5´→3´ direction in the presence of magnesium. The enzyme has a molecular weight of approximately 90,000 daltons as estimated from the predicted amino acid sequence and exhibits 3´→5´ exonuclease (proofreading) activity.
Pfu DNA Polymerase is recommended for use in PCR and primer extension reactions that require high fidelity.
Store at -20℃
| Application |
PCR and primer extension reactions |
| Blunt-end ligation |
| Precise amplification of bacterial sequences |
| Mutation research |
