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Many cell types currently used in research today will only grow and function in culture vessles when attached to a substrate. These cells are referred to as anchorage-dependent cells. Cell dissociation products have been adopted as a beneficial way to release these cells from their substrate for subculturing purposes. Numerous different parameters including tissue type, pH, media,temperature,incubation time,and concentration affect the outcome of adherent cell dissociation procedures.
Scraptase is desigend to be an alternative to porcine trypsin when used in conjuction with serum-free or serum-containing media. Scraptase contains a least amount of recombinant trypin and mixed chealating agents in dPBS for dissociation a wide range of adherent mammalian cells, inculding CHO,HEK293,A529, primary human keratinocytes, and embryonic stem cells.
Scraptase is very gentle on cells compared to porcine trypsin and other dissociation enzymes. It is recommended for use in studies requiring more intact cell surface proteins. Cells can be exposed to Scraptase for longer periods of time without the risk of damage associated with severe protein digestive enzyme like porcine trypsin. Scraptase is very stable with respect to temperature, pH and interference by serum components. In addition, activity is greatly reduced by dilution, dilution alone inactivates Scraptase, avoiding the need for trypsin inhibitor.
Store at -20℃
|Very gentle on cells||Contains a least amount of single recombinant enzyme. This reduces damage caused by cleavage from multiple enzyme activity in porcine
trypsin and other animal extract.
|Animal Origin Free(AOF)||Unlike porcine trypsin and other enzymes, Scraptase is free of animalderived components, and is formulated on dedicated animal origin free
|Stable||Scraptase is stable for 2 months at RT
Scraptase is stable for 12 months at 2-8oC
Scraptase is stable for 24 months at -20oC
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