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In an effort to produce a totally defined nutritinal source for cells, Morgan, Morton, and Parker, in 1950, developed what has become known as Medium 199. It was found that the growth of explanted tissue could be measured in Medium 199. However, long-term cultivation of cells required serum supplement. Currently, it is widely used in virology and vaccine production. It is also used in the culture of many non-transformed cells.
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|Menadione Sodium Bisulfite||No Menadione Sodium Bisulfite|
|Phenol Red||No Phenol Red|
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