Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Gly-Pro, which is found with high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation is
suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment.
Collagenase Type 5 is isolated from Clostridium histolyticum and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation,and organ perfusions. Collagenase Type 5 activity is guaranteed to be greater than 450 units/mg. Compared to other collagenase preparations, Collagenase Type 5 contains high levels of collagenase and caseinase activities, and is well suited for the digestion of fat, adrenal, and liver cells or tissues.
Store at 2-8℃
| Unit Definition |
One Unit releases one micromole of L-leucine equivalents from collagen in
5 hours at 37°C, pH 7.5 |
| Specification |
Collagenase activity: > 450 units/mg dry weight.
Caseinase activity: > 450 units/mg dry weight.
Clostripain activity: < 3.0 units/mg dry weight.
Trypsin activity: < 3.0 units/mg dry weight. |
| Activators/Cofactors |
Collagenase activity is stabilized by 0.1 mole calcium ions (Ca2+) per mole of
enzyme. Calcium ions also facilitate binding to the collagen molecule. Zinc ions
(Zn2+) are required for activity, but are tightly bound to the collagenase during
purification. Additional Zn2+ should not be necessary as long as no chelator is
added during digestion. |
| Inhibitors |
Ethylene glycol-bis(2-aminoethylether)-N,N,N¢,N¢-tetraacetic acid (EGTA)
Ethylenediaminetetraacetic acid (EDTA)
b-Thujaplicin (Hinokitiol)
Cysteine
2-mercaptoethanol
Glutathione (reduced form)
Thioglycolic acid, sodium
8-Hydroxyquinoline-5-sulfonate |
