amfiXpand PCR Premix is ready-to-use mixtures that include all of the reagents required for successful PCR, premixed and dried in single-use aliquots. amfiXpand PCR Premix is composed of a unique enzyme blend containing two thermostable
polymerases : Taq DNA polymerase and a thermostable polymerase with proofreading activity. amfiXpand PCR Premix is designed to amplify fragments up to 5 kb with high yield and fidelity, as well as with high specificity from episomal and genomic DNA.
amfiXpand PCR Premix contains red and yellow loading dyes to allow loading of PCR product directly on a gel after thermal cycling, minimizing pipetting steps and providing easy visualization of sample. The red dye runs in a range between 500bp (2% gels) and 1500bp (0.8% gels) and the yellow dye runs at less than 10bp. amfiXpand PCR Premix contains a fixed MgCl2 concentration of 1.75mM. However, higher concentrations may be achieved by adding additional MgCl2.
Store at -20℃
| Composition for Each Reaction |
1u/rxn amfiXpand Taq DNA Polymerase Mix, 10mM Tris-HCl(pH 8.8 at 25 ℃), 50mM KCl, 0.08% Nonidet P40, 1.75mM MgCl2, 0.2mM dGTP, 0.2mM dATP, 0.2mM dTTP, 0.2mM dCTP, stabilizers, and red and yellow loading dyes. |
| Contents |
8-Strip 0.2ml tube with individual attached cap containing amfiXpand PCR Master Mix. |
| Application |
Robust PCR
Hot Start PCR
Good for High GC Content PCR |
