amfiFusion High Fidelity PCR Master Mix combines amfiXpand Taq DNA Polymerase with high fidelity, proofreading DNA polymerase. This unique enzyme blend provides superior yields in both routine and challenging PCR. amfiFusion High Fidelity DNA Polymerase has 5’-3’ exonuclease acitivity of Taq DNA Polymerase as well as the 3’-5’ exonuclease activity of the proofreading DNA polymerase.
amfiFusion High Fidelity DNA Polymerase increase amplication fidelity up to 6 times over Taq DNA polymerase alone and allows for amplication of longer product size up to 19Kb. amfiFusion High Fidelity PCR Master Mix includes a single-strand DNA binding protein that is especially useful at blocking primers at lower temperatures making them unavailable for use by a polymerase. This DNA binding protein effectively blocks DNA synthesis from mis-priming events at lower temperatures.
In addition, amfiFusion High Fidelity PCR Master Mix contains red and yellow loading dyes to allow loading PCR product directly on a gel after thermal cycling, minimizing pipetting steps and providing easy visualization of sample.
Store at -20℃
| Long PCR |
Amplify up to 19 Kb |
| Direct Loading |
Load directly to gel from thermocycler No additional loading buffer needed Separates into 2 colors during gel electrophoresis |
| High Performance |
Higher Specificity/ Higher Yield / Higher Fidelity |
| High Fidelity |
amfiFusion High Fidelity DNA Polymerase has 5'-> 3' exonuclease activity of Taq DNA Polymerase as well as the 3'-> 5' exonuclease activity of the proofreading DNA polymerase. |
