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amfiSure qGreen Q-PCR Master Mix (2X) is a ready-to-use solution for quantitative real-time PCR and two-step real-time RT-PCR on most real-time PCR machines. The master mix includes amfiSure Hot-Start Taq DNA polymerase, dNTPs, qGreen Fluorescent DNA binding Dye (substitute for SYBR Green I dye) and rox passive dye in an optimized PCR buffer. amfiSure Hot-Start Taq DNA polymerase in combination Primers with an optimized buffer ensures PCR specificity and sensitivity.
qGreen Fluorescent intercalating dye allows for DNA detection and analysis without using sequence-specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of amfiSure qGreen Q-PCR Master Mix in real-time PCR ensure reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
- amfiSure qGreen & qProbe Q-PCR Master Mixes contain heat-labile chemical blocker which enables standard Taq DNA Polymerase to be inactivated securely during early PCR cycle and to be activated in 20 seconds at 95°C.
- amfiSure Hot StartTaq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.
- Improved processivity result in earlier Cq scores.
- Higher fluorescence detection across varying AT- and GC- rich targets.
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