Search term Western Blotting Reagent has been found in the following products: 17

Western Blotting Reagent

West-Q Femto Clean ECL Solution 100ml, W3680

  • Greater Sensitivity and Stable Signal
  • Very Low Background
  • Excellent Signal Duration
  • Compatible with Imaging System
  • Save on Antibody
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Cat No Size Price
W3680-010 100ml $ 210.00 Add to cart
W3680-020 200ml $ 310.00 Add to cart
W3680-040 400ml $ 520.00 Add to cart
Sensitivity Detect femtogram amounts of protein, stable signal 5 sec to 5 min exposure
Background Superior signal to noise, least background without compromising signal intensity.
Signal Duration 2 hour of usable light-output
Compatibility Easy to capture an image via a CCD camera or similar imaging system
Application Western detection of protein
West-Q Femto Clean ECL Solution is a non-isotopic,luminol- based chemiluminescent substrate designed for the rapid and sensitive detection of peroxidase labeled-conjugates. Provides maximum sensitivity in blotting application by allowing detection in the femto-gram range. Most other femto level ECL solution has high background it even has ultra-sensitive detection level. GenDEPOT’s West-Q Femto Clean ECL Solutionwas designed to make Western detection of protein easier by providing the highest level of sensitivity with the least amount of background in a chemilusmnescent reagent system.
  • GRP78 BiP(Protein#1) and actin(protein#2) were detected in HeLa Cell lysates. 5ug, 10ug, and 20ug of total proteins were seperated by SDS-PAGE Mouse anti-BiP(BD,610978) and rabbit anti-actin(Sigma, A2103) were used as primary antibodies with 1:1000 and 1:4,000 dilution, respectively. HRP-conjugated goat anti-mouse IgG and goat anti-rabbit IgG were used as secondary antibodies, respectively.

  • Purified GST was serially diluted from 5000 to 5 fg and electrophoresed on a 10% mini gel. The blot was then incubated with mouse anti-GST(200ug/ml)(Santa Cruz), SC-138) at 1:1000 dilution, followed by incubation with HRP-conjugated goat anti-mouse IgG(1mg/ml) at 1:5000 dilution.