As a component of sample buffer during protein extraction prior to Western blotting
As a component of assay buffer during protein extraction prior to radioimmunoassay
For the saturation of microplate well prior to double sandwich ELISA
As a component of biological buffer
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.