Search term Molecular Biology Enzyme has been found in the following products: 1

Molecular Biology Enzyme

T4 DNA Ligase, 1 Weiss u/㎕ ,T2622

  • Highly Purified
  • Isolated from a Recombinant Source
  • Active in restriction enzyme, PCR, and
  • RT buffers(when supplemented with ATP)
Collapse Store at -20℃
Cat No Size Price
T2622-020 200 units $ 0.00 Add to cart
T2622-100 5x200 units $ 330.00 Add to cart
T2622-200 10x200 units $ 520.00 Add to cart
T2622-500 25x200 units $ 850.00 Add to cart
Unit Definition One Weiss unit of T4 DNA Ligase converts 1 nmole of p32 from pyrophosphate into Norit-adsorbable material in 20 minutes at 37℃ in 33 mM Tris-acetate (pH 7.8), 66 mM potassium acetate, 10 mM magnesium acetate, 0.5 mM DTT, and 1 mM ATP. One Weiss unit equals approximately 200 cohesive- end ligation units.
Quality Control T4 DNA Ligase is functionally tested in cloning assays and is free of detectable contaminating DNA exo- and endo-nuclease and RNase activities.
Concentration 1 Weiss unit/㎕
Storage Buffer 50 mM Tris-HCl (pH 7.5), 0.1 M NaCl, 0.1 mM EDTA, 0.1% Triton X-100, and 1 mM DTT, 50% Glycerol
10X Reaction buffer composed of 400mM Tris-HCl, 100mM MgCl2, 100mM DTT, 5mM ATP (pH 7.8 at 25°C).
Application Cloning of restriction fragments
Joining linkers and adapters to blunt-ended DNA

T4 DNA Ligase is the most versatile and commonly used ligase for DNA cloning. This ATP-dependent enzyme covalently joins 5'-phosphates to 3'-hydroxy-lated termini at the blunt or compatible cohesive ends of double-stranded DNA fragments produced by restriction enzyme digestion or other enzymatic processes.

T4 DNA Ligase has no activity on single- stranded nucleic acids. Following a ligation reaction, T4 DNA Ligase may be inactivated by incubation at 65℃ for 10 minutes.