10 mM Tris-HCl , 100 mM KCl,1 mM DTT, 0.1 mM EDTA
0.5% EDTA,30% glycerol , pH 7.4 at 25°C
10X PCR Buffer
100mM Tris-HCl , 50 mM KCl,20mM (NH4)2SO4,
15mM MgCl2 0.5% NP-40, pH 8.8 at 25°C
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C
Taq DNA Polymerase is purified from E. coli. expressing a cloned Thermus aquaticus DNA polymerase gene. This enzyme has both a 5'->3' DNA polymerase and a 5'->3' exonuclease activity but lacks a 3'-> 5' exonuclease activity.