10 mM Tris-HCl , 100 mM KCl,1 mM DTT, 0.1 mM EDTA
0.5% EDTA,30% glycerol , pH 7.4 at 25°C
10X PCR Reaction Buffer
100mM Tris-HCl , 50 mM KCl,20mM (NH4)2SO4,
15mM MgCl2 0.5% NP-40, pH 8.8 at 25°C
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C
Taq DNA Polymerase is the industry standard for routine PCR. Taq with 10X PCR Buffer is available in economical extra-large pack sizes. GenDEPOT provides high quality recombinant Taq at an exceptional value. Taq DNA Polymerase is a thermostable DNA Polymerase that possesses a 5’->3’ polymerase activity and a
5’ flap endonuclease activity.
GenDEPOT has developed a uniquely efficient, automated manufacturing and purification process. We are therefore able to offer large quantities of highly purified enzyme at previously unheard of prices.
GenDEPOT’s Taq Polymerase Ultrapure is free from DNA, DNAse and pyrogens, and guranteed to deliver the most sensitive and relable PCR experience possible.