Tested by gel diffusion and ELISA techniques as applicable. This product reacts specifically with chicken IgY and may recognize other immuno-globulin types that have light chains in common with IgG. Reactivity to IgG subclasses has not been tested. Antibodies to chicken IgY may cross-react with immunoglobulins of other avian species if common binding sites are shared.
Suggested Working Concentration
Optimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution.
ELISA, Blotting, Histo/Cytochemical
Affinity purified antibody isolated from a pool of
serum from goats immunizedwith purified chicken
IgY was labeled with peroxidase by the periodate
methodof Nakane and Kawaoi (1).
Note: In many cases, the antibody may be diluted further than indicated.