Tested by gel diffusion and ELISA techniques as applicable. This product reacts specifically with rabbit IgG and may recognize other immunoglobulin types that have light chains in common with IgG. Reactivity to IgG subclasses has not been tested. Antibodies to rabbit IgG may cross-react with immunoglobulins of other mammalian species if common binding sites are shared. Cross-reactivity with human serum has been minimized with affinity procedures.
Suggested Working Concentration
Optimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution.
ELISA, Blotting,Histo/Cytochemical Procedures
Affinity purified antibody isolated from a pool of
serum from goats immunizedwith purified rabbit IgG