Tested by gel diffusion and ELISA techniques as applicable. This product reacts specifically with mouse IgG and may recognize other immunoglobulin types that have light chains in common with IgG. Reactivity to IgG subclasses has not been tested. Antibodies to mouse IgG may cross-react with immunoglobulins of other mammalian species if common binding sites are shared. Cross-reactivity with human serum has been minimized with affinity procedures.
Suggested Working Concentration
Optimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution.
1:1000 - 1:5000 (1.0 ug/mL - 0.2 ug/mL)
1:5000- 1:20000 (0.2 ug/mL - 0.05 ug/mL)
1:200 - 1:500 (5.0 ug/mL - 2.0 ug/mL)
ELIZA, Blotting, Histo/Cytochemical
Affinity purified antibody isolated from a pool of serum from goats immunized with purified mouse IgG was labeled with peroxidase by the periodate method of Nakane and Kawaoi.