RIPA II Cell Lysis Buffer(1X) Without EDTA - 100ml,contains Tris-HCl, pH 7.4, 50 mM, NaCl 150 mM ,Triton X-100 1%, Sodium deoxycholate 0.5% , SDS 0.1%
Suitable for molecular biology protein chemistry and biochemical applications
The RIPA Buffer is a reliable buffer used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. It enables the extraction of membrane, nuclear and cytoplasmic proteins and is compatible with many applications, including reporter assays, the BCA Protein Assay, immuno-assays and protein purification. Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail are also compatible with the RIPA Buffer and can be added before use to prevent proteolysis and maintain protein phosphorylation.