Total protein extraction from cultured cells and tissue samples.
Fast and Simple
No sonication or freeze-thaw cycles
room temperature process
Compatible with Downstream Applications
- 1D and 2D electrophoresis
- Western blotting and immunoprecipitation
- Affinity Purification
- EMSA, reporter gene assays and enzyme activity
ProteinSeeker Mammalian Cell Lysis Solution is a ready-to-use solution designed for extraction of native, non-denatured proteins from mammalian cultured cells or tissue samples. The procedure, which is based on lysis with mild detergents at room temperature, is fast and simple: no need for sonication, freeze thaw cycles or scraping of adherent cells. The procedure yields proteins in their native, non-denatured functional state. Therefore, isolated proteins can be used directly in many proteomic and enzymatic activity applications, including immunoprecipitation, affinity purification, reporter gene assays and electrophoretic mobility shift assays (EMSA). Isolated proteins are also compatible with quantification assays such as Bradford and Lowry.