Mini-gel: 5 - 10 µL per well (0.75-1.5 mm thick)
Large gel: 10 - 20 µL per well (0.75-1.5mm thick)
Thaw the ladder at room temperature. Do not boil protein marker. It is ready-to-use.
Xpert Unstained Protein Marker is a mixture of 12 recombinant, highly purified proteins, which resolve into clearly identifiable sharp bands from 10-250 kDa when analyzed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250. This ladder is intended for use as a precise size standard when performing SDS-PAGE to calculate the molecular weight of a protein of interest. The 25 and 80 kDa bands have triple the intensity of the other proteins and serve as refer-ence indicators. The molecular weights of the Protein Ladder are confirmed by sequencing, mass spectrometry and migration in a Laemmli SDS-Page Tris-Glycine system.