Search term Protein Assay Reagent has been found in the following products: 8

Protein Assay Reagent

Bradford(Coomassie) Protein Assay Plus Reagents, P7201

  • Stable, Ready-to-Use
  • Linear Responses - 1 ~ 2000 ug/ml
  • Easy to use - Single Reagent
  • Improved Linearity and Response
  • Uniformity
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Cat No Size Price
P7201-050 450ml $ 96.00 Add to cart
Maximum concentrations that are compatible Amino acids : 1mM
Ammonium sulfate : 1M
Ampholytes : 0.5%
Ascorbic acid : 50mM
Boric acid : 1mM
Brij® 35 : 0.06%
CHAPS : 0.5%
CHAPSO : 0.5%
Citrate : 0.05%
Cysteine : 10mM
Deoxycholate : 0.1%
DMSO : 10%
DNA : 1mg/ml
Maximum concentrations that are compatible DTT : 1M
EDTA : 100mM
EGTA : 50mM
Ethanol : 10%
Glucose : 1M
Glycerol : 10%
Glycine : 0.1M
Guanidine.HCl : 6M
HEPES : 0.1M
2-mercaptoethanol : 1M
Methanol : 10%
MES : 0.7M
Nonidet® P-40 : 0.5%
Maximum concentrations that are compatible Phenol : 5%
Sodium azide : 0.5%
Sodium chloride : 6M
Sodium dodecyl sulfate (SDS) : 0.015%
Sodium hydroxide : 0.1M
Sodium phosphate : 0.1M
Sucrose : 25%
Tris : 2M
Triton® X-100 : 0.06%
tRNA : 0.35mg/ml
Tween® 20 : 0.03%
Urea : 3M
Bradford(Coomassie) Protein Assay Plus Reagent is designed for rapid and accurate estimation of total protein concentration in solution. The bradford (Coomassie) protein assay relies on the formation of complexes between coomassie brilliant Blue G-250 dye and proteins in solution. This binding causes a shift in the absorption maximum of the dye from 465 nm to 595 nm. The concentration of the protein sample is determined by referencing the absorption to a series of standard protein dilutions assayed in parallel.

The quantification procedure is simple and rapid. The protein sample is mixed with the reagent at room temperature, the absorbance of the solutions is measured at 595 nm. The absorption at 595nm is proportional to the amount of protein present in the solution. The dye binding process is complete in approximately 5 min with color stability for 1 hour. The working range of the Bradford(Coomassie) Protein Assay Plus Reagent is 1 ~ 2000 ug/ml of protein.