amfiSure Advanced PCR Master Mix is ready-to-use mixtures that include all of the reagents needed for PCR. amfiSure Advanced PCR Master Mix provides superior yields in both routine and challenging PCR application. Using advanced amfiSure PCR Master Mix in routine and challenging PCR reduces smearing and virtually eliminates unwanted background artifacts. amfiSure Advanced PCR Master Mix has been optimized for amplifying DNA template up to 3kb. amfiSure Advanced PCR Master Mix contains red and yellow loading dyes to allow loading of PCR product directly on a gel after thermal cycling, minimizing pipetting steps and providing easy visualization of sample.
The red dye runs in a range between 500bp (2% gel) and 1500bp (0.8% gel) and the yellow dye runs at less than 10bp. amfiSure Advanced PCR Master Mix contains a fixed MgCl2concentration of 1.5 mM. However, higher concentra-tions of MgCl2may be achieved by adding additional MgCl2.