-amfiFusion High Fidelity DNA Polymerase
-amfiFusion 2X Reaction Buffer
amfiFusion High Fidelity DNA Polymerase combines amfiXpand Taq DNA Polymerase with high fidelity, proofreading DNA polymerase. This unique enzyme blend provides superior yields in both routine and challenging PCR. amfiFusion High Fidelity DNA Polymerase has 5’-3’ exonuclease acitivity of Taq DNA Polymerase as well as the 3’-5’ exonuclease activity of the proofreading DNA polymerase.
amfiFusion High Fidelity DNA Polymerase increase amplication fidelity up to 6 times over Taq DNA polymerase alone and allows for amplication of longer product size up to 19Kb. amfiFusion 2X Reaction Buffer includes a single strand DNA binding protein that is especially useful at blocking primers at lower temperatures making them unavailable for use by a polymerase. This DNA binding protein that is unavailable for use by a polymerase. This DNA binding protein effectively blocks DNA synthesis from mis-priming events at lower temperatures.
In addition amfiFusion 2X Reaction Buffer including dNTP’s and Mg++ minimizes reagent handling steps and reduces the risk of contamination. For added convenience, amfiFusion High Fidelity DNA Polymerase contains red and yellow loading dyes to allow loading PCR product directly on a gel after thermal cycling, minimizing pipetting steps and providing easy visualization of sample.